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FORLAB Ham's F-12/F12K Cell Culture Media
Ham's F-12 medium was designed for serum-free single cell spreading of Chinese hamster ovary (CHO) cells. Since then, F-12 has been used for serum-free growth of CHO cultures as well as serum-added growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. It is particularly suitable for single-cell and clonogenic cultures at low serum levels and is also widely used for the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells, when serum is added. In addition, Ham's F-12 is mixed with DMEM in equal volume to obtain a more nutritious DMEM/F12 medium, which is more widely used.
Ham's F-12K medium is based on Ham's F-12 with an increased concentration of amino acids and pyruvate, a reduced amount of glucose, and a modified salt composition and content (Konigsbergs). Ham's F-12K medium is used for the cultivation of primary human hepatocytes as well as differentiated rat and chicken cells.
It contains many amino acids, vitamins, inorganic salts, and other ingredients required for the culture of many types of cells, but does not contain proteins, lipids, or any growth factors; therefore, this product should be used with serum or serum-free additives.
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Product information
FORLAB Ham's F-12 Medium
Order number Description Characteristic Ingredients Contains(+) Nope（-） FL10015 Ham's F-12 medium This product is filtered and sterilised 1.8g/L D-glucose HEPES Endotoxin: ≤1EU/mL L-Glutamine pH value: 7.0-7.4 Phenol Red Storage conditions: 2 - 8 ℃ stored away from light Sodium pyruvate Valid for 12 months 500ml/bottle FORLAB Ham's F-12K Medium
Order number Description Characteristic Ingredients Contains(+) Nope（-） FL10115 Ham's F-12K medium This product is a filtration and sterilization product 1.26 g/L D-glucose HEPES Endotoxin: ≤1EU/mL L-glutamine pH: 7.0-7.4 Phenol red Storage conditions: 2 - 8 °C protected from light Sodium pyruvate Valid for 12 months 500ml/bottle -
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